ssr_primer
Primer Details for TRINITY_DN77420_c1_g1_i6 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCTTGATCCTTGCCCAT | GCAAACAGGACATGGCTTGG | Start: 1604 |
| Tm | 59.958 | 60.038 | End: 1770 |
| Size | 20 | 20 | 167 |
| Primer 2 details |
|---|
| Sequence | CGAAGGTTAGACTGCAGGCA | TCGCAAAACAATGCAGGCAA | Start: 27 |
| Tm | 60.037 | 59.896 | End: 160 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | CGAAGGTTAGACTGCAGGCA | TTCGCAAAACAATGCAGGCA | Start: 27 |
| Tm | 60.037 | 59.896 | End: 161 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | CGTTTGCCTTGATCCTTGCC | GCAAACAGGACATGGCTTGG | Start: 1601 |
| Tm | 60.109 | 60.038 | End: 1770 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | TTGCCTTGATCCTTGCCCAT | CGAAGCAAACAGGACATGGC | Start: 1604 |
| Tm | 59.958 | 60.11 | End: 1774 |
| Size | 20 | 20 | 171 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCTTGATCCTTGCCCAT | GCAAACAGGACATGGCTTGG | Start: 1604 |
| Tm | 59.958 | 60.038 | End: 1770 |
| Size | 20 | 20 | 167 |
| Primer 2 details |
|---|
| Sequence | CGAAGGTTAGACTGCAGGCA | TCGCAAAACAATGCAGGCAA | Start: 27 |
| Tm | 60.037 | 59.896 | End: 160 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | CGAAGGTTAGACTGCAGGCA | TTCGCAAAACAATGCAGGCA | Start: 27 |
| Tm | 60.037 | 59.896 | End: 161 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | CGTTTGCCTTGATCCTTGCC | GCAAACAGGACATGGCTTGG | Start: 1601 |
| Tm | 60.109 | 60.038 | End: 1770 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | TTGCCTTGATCCTTGCCCAT | CGAAGCAAACAGGACATGGC | Start: 1604 |
| Tm | 59.958 | 60.11 | End: 1774 |
| Size | 20 | 20 | 171 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCTTGATCCTTGCCCAT | GCAAACAGGACATGGCTTGG | Start: 1604 |
| Tm | 59.958 | 60.038 | End: 1770 |
| Size | 20 | 20 | 167 |
| Primer 2 details |
|---|
| Sequence | CGAAGGTTAGACTGCAGGCA | TCGCAAAACAATGCAGGCAA | Start: 27 |
| Tm | 60.037 | 59.896 | End: 160 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | CGAAGGTTAGACTGCAGGCA | TTCGCAAAACAATGCAGGCA | Start: 27 |
| Tm | 60.037 | 59.896 | End: 161 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | CGTTTGCCTTGATCCTTGCC | GCAAACAGGACATGGCTTGG | Start: 1601 |
| Tm | 60.109 | 60.038 | End: 1770 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | TTGCCTTGATCCTTGCCCAT | CGAAGCAAACAGGACATGGC | Start: 1604 |
| Tm | 59.958 | 60.11 | End: 1774 |
| Size | 20 | 20 | 171 |
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