ssr_primer
Primer Details for TRINITY_DN77373_c0_g1_i1 |
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| (A)48: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACAGTCGGCTCATCCTCT | AACAACTTAGTCGGCGCAGA | Start: 1733 |
| Tm | 60.034 | 59.967 | End: 1961 |
| Size | 20 | 20 | 229 |
| Primer 2 details |
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| Sequence | ACGAAGAGGGGGATGATCCA | TCTTCGACTTTGGTCTCCGC | Start: 956 |
| Tm | 60.031 | 60.039 | End: 1094 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
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| Sequence | TCTGCGCCGACTAAGTTGTT | AGGAAGGCGAGGGACTACTT | Start: 1942 |
| Tm | 59.967 | 59.959 | End: 2045 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | GACAGTCGGCTCATCCTCTG | AACAACTTAGTCGGCGCAGA | Start: 1734 |
| Tm | 59.898 | 59.967 | End: 1961 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
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| Sequence | TCTGCGCCGACTAAGTTGTT | GGATAGCCCAGGAGGTGAGA | Start: 1942 |
| Tm | 59.967 | 60.105 | End: 2121 |
| Size | 20 | 20 | 180 |
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