ssr_primer
Primer Details for TRINITY_DN77360_c3_g4_i2 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGACTGGACTTGCCCTCC | TGACTTACACAAGCGCTCGT | Start: 1949 |
| Tm | 59.89 | 59.968 | End: 2212 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
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| Sequence | AGAACGCCTCAAAGCACTGA | GCAAACCTGTCTCCCCTCTC | Start: 971 |
| Tm | 59.893 | 60.036 | End: 1114 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
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| Sequence | GCAATTGGGAGTCGCAGAAC | GCAAACCTGTCTCCCCTCTC | Start: 956 |
| Tm | 59.831 | 60.036 | End: 1114 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | CTTCTGTTGGTGGTCGGGAA | ACCTTCGAACAACCTTGCCA | Start: 2172 |
| Tm | 59.892 | 60.107 | End: 2282 |
| Size | 20 | 20 | 111 |
| Primer 5 details |
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| Sequence | AGAACGCCTCAAAGCACTGA | CAAGCAAACCTGTCTCCCCT | Start: 971 |
| Tm | 59.893 | 59.889 | End: 1117 |
| Size | 20 | 20 | 147 |
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