ssr_primer
Primer Details for TRINITY_DN77346_c1_g5_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGCACGGAGAAAGACTC | AGGATCAGCTTGGGTTGACG | Start: 989 |
| Tm | 59.965 | 60.036 | End: 1200 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
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| Sequence | GACTTGGGCACGGAGAAAGA | AGGATCAGCTTGGGTTGACG | Start: 986 |
| Tm | 59.965 | 60.036 | End: 1200 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | CGTCAACCCAAGCTGATCCT | TCACTCTCTCCTAGTGCGCT | Start: 1181 |
| Tm | 60.036 | 60.035 | End: 1369 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
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| Sequence | CTTGGGCACGGAGAAAGACT | AGGATCAGCTTGGGTTGACG | Start: 988 |
| Tm | 59.965 | 60.036 | End: 1200 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
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| Sequence | AGCTCTCAAGTGACTTGGGC | AGGATCAGCTTGGGTTGACG | Start: 975 |
| Tm | 59.964 | 60.036 | End: 1200 |
| Size | 20 | 20 | 226 |
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