ssr_primer
Primer Details for TRINITY_DN77336_c7_g1_i1 |
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| (C)10acccccctttttaggccggagacctaatttacaactga(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTTGAAGGAGGCCCCCG | TGGCTGCAGATGCTTAGACA | Start: 5 |
| Tm | 60.615 | 59.385 | End: 125 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
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| Sequence | CCCTTTTTAGGCCGGAGACC | CAGTTCGGTCCCTAATCACGT | Start: 46 |
| Tm | 60.394 | 59.796 | End: 183 |
| Size | 20 | 21 | 138 |
| Primer 3 details |
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| Sequence | CCCCTTTTTAGGCCGGAGAC | CAGTTCGGTCCCTAATCACGT | Start: 45 |
| Tm | 60.394 | 59.796 | End: 183 |
| Size | 20 | 21 | 139 |
| Primer 4 details |
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| Sequence | ATTTTGAAGGAGGCCCCCG | TGGCTGCAGATGCTTAGACA | Start: 6 |
| Tm | 60 | 59.385 | End: 125 |
| Size | 19 | 20 | 120 |
| Primer 5 details |
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| Sequence | AATTTTGAAGGAGGCCCCCG | TTGGCTGCAGATGCTTAGACA | Start: 5 |
| Tm | 60.615 | 59.997 | End: 126 |
| Size | 20 | 21 | 122 |
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