ssr_primer
Primer Details for TRINITY_DN77328_c2_g2_i1 |
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| (T)12cctttagattttagtgcactttagtgggtttc(T)10(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTCACTCTCAGTTGTGT | GCTGACATTACCACCAGCCT | Start: 2200 |
| Tm | 59.967 | 60.035 | End: 2407 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
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| Sequence | AATGAGCCAGTCGATAGCCG | CTCGTTGGGGTCATCGTTCA | Start: 446 |
| Tm | 59.967 | 60.038 | End: 652 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
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| Sequence | TGAACGATGACCCCAACGAG | GAAGTGAGACCCAGTCGCAA | Start: 633 |
| Tm | 60.038 | 59.966 | End: 754 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
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| Sequence | CAAGGAAACCCCGGGAATGA | ACACAACTGAGAGTGAGGCG | Start: 1967 |
| Tm | 59.961 | 59.967 | End: 2219 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
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| Sequence | CGATCTGGGGTACGTACGTG | AACCATCCTGCAGCTGGAAA | Start: 54 |
| Tm | 59.971 | 59.887 | End: 264 |
| Size | 20 | 20 | 211 |
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