ssr_primer
Primer Details for TRINITY_DN77302_c6_g1_i1 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTGGATGAATGCCGAAT | GCGTACGGATTTCCTGACCA | Start: 1563 |
| Tm | 60.107 | 60.109 | End: 1772 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | ACGGTGGATGAATGCCGAAT | TGCGTACGGATTTCCTGACC | Start: 1563 |
| Tm | 60.107 | 60.109 | End: 1773 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | TAATGAGGTAGTGCGCCCAC | ACTGTTGATGACTACGCGCA | Start: 2017 |
| Tm | 59.823 | 60.039 | End: 2147 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | TGCGCGTAGTCATCAACAGT | ACCCACGGTTCATTCGTTGT | Start: 2128 |
| Tm | 60.039 | 60.179 | End: 2386 |
| Size | 20 | 20 | 259 |
| Primer 5 details |
|---|
| Sequence | CGAAACCGAAAAGTGGGCAG | CCCCTGGCGCTAAATAAGGT | Start: 1125 |
| Tm | 60.04 | 59.817 | End: 1356 |
| Size | 20 | 20 | 232 |
(T)18atttattta(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTGGATGAATGCCGAAT | GCGTACGGATTTCCTGACCA | Start: 1563 |
| Tm | 60.107 | 60.109 | End: 1772 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | ACGGTGGATGAATGCCGAAT | TGCGTACGGATTTCCTGACC | Start: 1563 |
| Tm | 60.107 | 60.109 | End: 1773 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | TAATGAGGTAGTGCGCCCAC | ACTGTTGATGACTACGCGCA | Start: 2017 |
| Tm | 59.823 | 60.039 | End: 2147 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | TGCGCGTAGTCATCAACAGT | ACCCACGGTTCATTCGTTGT | Start: 2128 |
| Tm | 60.039 | 60.179 | End: 2386 |
| Size | 20 | 20 | 259 |
| Primer 5 details |
|---|
| Sequence | CGAAACCGAAAAGTGGGCAG | CCCCTGGCGCTAAATAAGGT | Start: 1125 |
| Tm | 60.04 | 59.817 | End: 1356 |
| Size | 20 | 20 | 232 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTGGATGAATGCCGAAT | GCGTACGGATTTCCTGACCA | Start: 1563 |
| Tm | 60.107 | 60.109 | End: 1772 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | ACGGTGGATGAATGCCGAAT | TGCGTACGGATTTCCTGACC | Start: 1563 |
| Tm | 60.107 | 60.109 | End: 1773 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | TAATGAGGTAGTGCGCCCAC | ACTGTTGATGACTACGCGCA | Start: 2017 |
| Tm | 59.823 | 60.039 | End: 2147 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | TGCGCGTAGTCATCAACAGT | ACCCACGGTTCATTCGTTGT | Start: 2128 |
| Tm | 60.039 | 60.179 | End: 2386 |
| Size | 20 | 20 | 259 |
| Primer 5 details |
|---|
| Sequence | CGAAACCGAAAAGTGGGCAG | CCCCTGGCGCTAAATAAGGT | Start: 1125 |
| Tm | 60.04 | 59.817 | End: 1356 |
| Size | 20 | 20 | 232 |
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