ssr_primer
Primer Details for TRINITY_DN77273_c0_g1_i3 |
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| (A)13cgagtagagacatggccgtcttatttttctt(A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGTAGAGACATGGCCGTC | GGCGTTGAGAGCTTTTTGCA | Start: 1079 |
| Tm | 59.97 | 59.969 | End: 1258 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
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| Sequence | CGAGTAGAGACATGGCCGTC | TGGCGTTGAGAGCTTTTTGC | Start: 1079 |
| Tm | 59.97 | 59.969 | End: 1259 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
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| Sequence | CGAGTAGAGACATGGCCGTC | TTCTCGCACCCTGGGTTAAC | Start: 1079 |
| Tm | 59.97 | 59.964 | End: 1301 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
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| Sequence | CGAGTAGAGACATGGCCGTC | ATCGGTTTGCGTGCTCTACA | Start: 1079 |
| Tm | 59.97 | 60.038 | End: 1325 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
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| Sequence | CGGCAAGAGCACTCCTTACA | CTCTTGGAGCCCCCTAGACT | Start: 1840 |
| Tm | 60.037 | 60.032 | End: 2073 |
| Size | 20 | 20 | 234 |
(G)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGTAGAGACATGGCCGTC | GGCGTTGAGAGCTTTTTGCA | Start: 1079 |
| Tm | 59.97 | 59.969 | End: 1258 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | CGAGTAGAGACATGGCCGTC | TGGCGTTGAGAGCTTTTTGC | Start: 1079 |
| Tm | 59.97 | 59.969 | End: 1259 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | CGAGTAGAGACATGGCCGTC | TTCTCGCACCCTGGGTTAAC | Start: 1079 |
| Tm | 59.97 | 59.964 | End: 1301 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | CGAGTAGAGACATGGCCGTC | ATCGGTTTGCGTGCTCTACA | Start: 1079 |
| Tm | 59.97 | 60.038 | End: 1325 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | CGGCAAGAGCACTCCTTACA | CTCTTGGAGCCCCCTAGACT | Start: 1840 |
| Tm | 60.037 | 60.032 | End: 2073 |
| Size | 20 | 20 | 234 |
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