ssr_primer
Primer Details for TRINITY_DN77269_c1_g1_i15 |
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| (T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGTTTCTTGTCCTCCTCC | CTAGACCAGCCCTCCTCACT | Start: 56 |
| Tm | 60.036 | 60.033 | End: 221 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
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| Sequence | TTCTCGCTCCTGGGTTTCAC | AGCTAAGCGCCTGTTCACTT | Start: 1233 |
| Tm | 59.965 | 59.965 | End: 1428 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
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| Sequence | GTTCTCGCTCCTGGGTTTCA | AGCTAAGCGCCTGTTCACTT | Start: 1232 |
| Tm | 59.965 | 59.965 | End: 1428 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
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| Sequence | AGTCGCCATGTGCATTCTCA | ATCTCCAGTCCGGACTTCCA | Start: 1751 |
| Tm | 60.036 | 59.959 | End: 1913 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | GCAACCTACAACTGCCTCCT | ATCTCCAGTCCGGACTTCCA | Start: 1778 |
| Tm | 59.963 | 59.959 | End: 1913 |
| Size | 20 | 20 | 136 |
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