ssr_primer
Primer Details for TRINITY_DN77250_c0_g1_i1 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCATCAGACGCCGGAAGAC | TGAGCAACGTTGACATGGGT | Start: 45 |
| Tm | 59.897 | 60.179 | End: 186 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
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| Sequence | ACTTCGATCATCAGACGCCG | TGAGCAACGTTGACATGGGT | Start: 39 |
| Tm | 60.249 | 60.179 | End: 186 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
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| Sequence | GATCATCAGACGCCGGAAGA | TGAGCAACGTTGACATGGGT | Start: 44 |
| Tm | 59.614 | 60.179 | End: 186 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
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| Sequence | ATCATCAGACGCCGGAAGAC | CTGAGCAACGTTGACATGGG | Start: 45 |
| Tm | 59.897 | 59.481 | End: 187 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
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| Sequence | ACTTCGATCATCAGACGCCG | CTGAGCAACGTTGACATGGG | Start: 39 |
| Tm | 60.249 | 59.481 | End: 187 |
| Size | 20 | 20 | 149 |
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