ssr_primer
Primer Details for TRINITY_DN77238_c8_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCCGCATGACACTTAAGA | GCAGTCCCAAAGAACTCCTCA | Start: 146 |
| Tm | 60.038 | 59.928 | End: 300 |
| Size | 20 | 21 | 155 |
| Primer 2 details |
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| Sequence | TGAGGAGTTCTTTGGGACTGC | CCGGATAAGCAATGGACGGA | Start: 280 |
| Tm | 59.928 | 59.894 | End: 493 |
| Size | 21 | 20 | 214 |
| Primer 3 details |
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| Sequence | TGAGGAGTTCTTTGGGACTGC | TGTCGTGCCGGATAAGCAAT | Start: 280 |
| Tm | 59.928 | 60.108 | End: 500 |
| Size | 21 | 20 | 221 |
| Primer 4 details |
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| Sequence | TGAGGAGTTCTTTGGGACTGC | ATGTCGTGCCGGATAAGCAA | Start: 280 |
| Tm | 59.928 | 60.108 | End: 501 |
| Size | 21 | 20 | 222 |
| Primer 5 details |
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| Sequence | TGAGGAGTTCTTTGGGACTGC | AATGTCGTGCCGGATAAGCA | Start: 280 |
| Tm | 59.928 | 60.108 | End: 502 |
| Size | 21 | 20 | 223 |
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