Primer Details for TRINITY_DN77217_c4_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAAGAAACAAGAAGAGAAGGAGA | TCCTCGTCTTCTCTTTTTCCCA | Start: 62 |
| Tm | 58.346 | 59.03 | End: 163 |
| Size | 25 | 22 | 102 |
| Primer 2 details |
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| Sequence | ACAAAGAAACAAGAAGAGAAGGAG | TCCTCGTCTTCTCTTTTTCCCA | Start: 62 |
| Tm | 57.066 | 59.03 | End: 163 |
| Size | 24 | 22 | 102 |
| Primer 3 details |
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| Sequence | GGAAACAAAGAAACAAGAAGAGAAGG | TCCTCGTCTTCTCTTTTTCCCA | Start: 58 |
| Tm | 59.025 | 59.03 | End: 163 |
| Size | 26 | 22 | 106 |
| Primer 4 details |
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| Sequence | AGGGGAGAGAAAAAGAAGAAAAATGA | TCCTCGTCTTCTCTTTTTCCCA | Start: 0 |
| Tm | 58.931 | 59.03 | End: 163 |
| Size | 26 | 22 | 164 |
| Primer 5 details |
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| Sequence | ACAAAGAAACAAGAAGAGAAGGAGA | CCTCGTCTTCTCTTTTTCCCA | Start: 62 |
| Tm | 58.346 | 57.597 | End: 162 |
| Size | 25 | 21 | 101 |
