ssr_primer
Primer Details for TRINITY_DN77150_c4_g5_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGTTTGGCCGCCACTAAA | AAGAGGACTGCACTTGGACG | Start: 1173 |
| Tm | 59.89 | 59.966 | End: 1355 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | AGAGTTTGGCCGCCACTAAA | GGCCGTGGAAGAGTGTTACA | Start: 1173 |
| Tm | 59.89 | 59.966 | End: 1285 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | TTCTCGGCACTTTGAACCCA | GAAAGGAGCCTGTTCCGTGA | Start: 1849 |
| Tm | 59.818 | 59.965 | End: 1978 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
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| Sequence | TTCTCGGCACTTTGAACCCA | AAGGAGCCTGTTCCGTGATG | Start: 1849 |
| Tm | 59.818 | 60.036 | End: 1976 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
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| Sequence | TTCTCGGCACTTTGAACCCA | GAGCCTGTTCCGTGATGTCA | Start: 1849 |
| Tm | 59.818 | 60.037 | End: 1973 |
| Size | 20 | 20 | 125 |
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