ssr_primer
Primer Details for TRINITY_DN77150_c4_g1_i3 |
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| (A)32: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGGCAACTGTGAGCAAGA | TCGCCCACTTCAAGAACCAA | Start: 655 |
| Tm | 60.107 | 59.818 | End: 800 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
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| Sequence | TGTGGCAACTGTGAGCAAGA | TTCGCCCACTTCAAGAACCA | Start: 655 |
| Tm | 60.107 | 59.818 | End: 801 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
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| Sequence | TGGTTCTTGAAGTGGGCGAA | ACACAGTGAAGCAGCTGGAA | Start: 782 |
| Tm | 59.818 | 59.819 | End: 1029 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
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| Sequence | TTGGTTCTTGAAGTGGGCGA | ACACAGTGAAGCAGCTGGAA | Start: 781 |
| Tm | 59.818 | 59.819 | End: 1029 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
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| Sequence | GGGAAAAGTGAGGCAGAGCT | AGGCAGCTAAGCTTTGCTCT | Start: 2260 |
| Tm | 59.963 | 59.672 | End: 2432 |
| Size | 20 | 20 | 173 |
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