ssr_primer
Primer Details for TRINITY_DN77149_c7_g2_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAAGGCACCAACTTCCAGC | CCAACCTGGGTCCTGTGTAC | Start: 278 |
| Tm | 59.967 | 59.964 | End: 474 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | ACCAAAGGCACCAACTTCCA | CCAACCTGGGTCCTGTGTAC | Start: 276 |
| Tm | 60.033 | 59.964 | End: 474 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | GTACACAGGACCCAGGTTGG | GGCCTGAGATGCTCCTTTGT | Start: 455 |
| Tm | 59.964 | 60.035 | End: 584 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GTACACAGGACCCAGGTTGG | TTGCCGAAAGATCCCACTCC | Start: 455 |
| Tm | 59.964 | 60.035 | End: 604 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | ACAAAGGAGCATCTCAGGCC | TCCACCCTGCAAAGCGTTAT | Start: 565 |
| Tm | 60.035 | 59.962 | End: 667 |
| Size | 20 | 20 | 103 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAAGGCACCAACTTCCAGC | CCAACCTGGGTCCTGTGTAC | Start: 278 |
| Tm | 59.967 | 59.964 | End: 474 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | ACCAAAGGCACCAACTTCCA | CCAACCTGGGTCCTGTGTAC | Start: 276 |
| Tm | 60.033 | 59.964 | End: 474 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | GTACACAGGACCCAGGTTGG | GGCCTGAGATGCTCCTTTGT | Start: 455 |
| Tm | 59.964 | 60.035 | End: 584 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GTACACAGGACCCAGGTTGG | TTGCCGAAAGATCCCACTCC | Start: 455 |
| Tm | 59.964 | 60.035 | End: 604 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | ACAAAGGAGCATCTCAGGCC | TCCACCCTGCAAAGCGTTAT | Start: 565 |
| Tm | 60.035 | 59.962 | End: 667 |
| Size | 20 | 20 | 103 |
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