ssr_primer
Primer Details for TRINITY_DN77129_c2_g2_i6 |
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| (T)13atttttcaaacagcaaattgcgaactcttctaaaatgcatc(T)26: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTAGGGGGCTCTTAGCTT | CCAACAGTGTCAGATTGTGCG | Start: 772 |
| Tm | 60.031 | 60.07 | End: 898 |
| Size | 20 | 21 | 127 |
| Primer 2 details |
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| Sequence | TGGCACTATCTAGCAGCGATG | ACAGAAGCTAAGAGCCCCCT | Start: 674 |
| Tm | 60 | 60.253 | End: 795 |
| Size | 21 | 20 | 122 |
| Primer 3 details |
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| Sequence | AGCTAGGGGGCTCTTAGCTT | TGGTACACCAACACCAACAGT | Start: 772 |
| Tm | 60.031 | 59.718 | End: 911 |
| Size | 20 | 21 | 140 |
| Primer 4 details |
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| Sequence | GGCACTGGATCCTCAATGTCA | ACAGAAGCTAAGAGCCCCCT | Start: 582 |
| Tm | 60.065 | 60.253 | End: 795 |
| Size | 21 | 20 | 214 |
| Primer 5 details |
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| Sequence | GCACTATCTAGCAGCGATGC | ACAGAAGCTAAGAGCCCCCT | Start: 676 |
| Tm | 58.933 | 60.253 | End: 795 |
| Size | 20 | 20 | 120 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTAGGGGGCTCTTAGCTT | CCAACAGTGTCAGATTGTGCG | Start: 772 |
| Tm | 60.031 | 60.07 | End: 898 |
| Size | 20 | 21 | 127 |
| Primer 2 details |
|---|
| Sequence | TGGCACTATCTAGCAGCGATG | ACAGAAGCTAAGAGCCCCCT | Start: 674 |
| Tm | 60 | 60.253 | End: 795 |
| Size | 21 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | AGCTAGGGGGCTCTTAGCTT | TGGTACACCAACACCAACAGT | Start: 772 |
| Tm | 60.031 | 59.718 | End: 911 |
| Size | 20 | 21 | 140 |
| Primer 4 details |
|---|
| Sequence | GGCACTGGATCCTCAATGTCA | ACAGAAGCTAAGAGCCCCCT | Start: 582 |
| Tm | 60.065 | 60.253 | End: 795 |
| Size | 21 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | GCACTATCTAGCAGCGATGC | ACAGAAGCTAAGAGCCCCCT | Start: 676 |
| Tm | 58.933 | 60.253 | End: 795 |
| Size | 20 | 20 | 120 |
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