ssr_primer
Primer Details for TRINITY_DN77100_c3_g2_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCTCTCTCTCTGCCGAA | AGAAGGCTGCGGAAAAAGGA | Start: 49 |
| Tm | 60.251 | 59.89 | End: 148 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | TGGGCCGTTTTGACATCACT | AGCTTTCATGATGCCCTCCG | Start: 466 |
| Tm | 60.179 | 60.465 | End: 650 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | ACACCTCTCTCTCTGCCGAA | AGCGGAGAGTTAGACGAAGC | Start: 49 |
| Tm | 60.251 | 59.547 | End: 185 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | CGGAGGGCATCATGAAAGCT | TTTTCACGCCGGCTTTTCAC | Start: 631 |
| Tm | 60.465 | 60.249 | End: 791 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
|---|
| Sequence | TCCTTTTTCCGCAGCCTTCT | GGTGGTCTGGAACAAACACG | Start: 129 |
| Tm | 59.89 | 59.339 | End: 391 |
| Size | 20 | 20 | 263 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCTCTCTCTCTGCCGAA | AGAAGGCTGCGGAAAAAGGA | Start: 49 |
| Tm | 60.251 | 59.89 | End: 148 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | TGGGCCGTTTTGACATCACT | AGCTTTCATGATGCCCTCCG | Start: 466 |
| Tm | 60.179 | 60.465 | End: 650 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | ACACCTCTCTCTCTGCCGAA | AGCGGAGAGTTAGACGAAGC | Start: 49 |
| Tm | 60.251 | 59.547 | End: 185 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | CGGAGGGCATCATGAAAGCT | TTTTCACGCCGGCTTTTCAC | Start: 631 |
| Tm | 60.465 | 60.249 | End: 791 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
|---|
| Sequence | TCCTTTTTCCGCAGCCTTCT | GGTGGTCTGGAACAAACACG | Start: 129 |
| Tm | 59.89 | 59.339 | End: 391 |
| Size | 20 | 20 | 263 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCTCTCTCTCTGCCGAA | AGAAGGCTGCGGAAAAAGGA | Start: 49 |
| Tm | 60.251 | 59.89 | End: 148 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | TGGGCCGTTTTGACATCACT | AGCTTTCATGATGCCCTCCG | Start: 466 |
| Tm | 60.179 | 60.465 | End: 650 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | ACACCTCTCTCTCTGCCGAA | AGCGGAGAGTTAGACGAAGC | Start: 49 |
| Tm | 60.251 | 59.547 | End: 185 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | CGGAGGGCATCATGAAAGCT | TTTTCACGCCGGCTTTTCAC | Start: 631 |
| Tm | 60.465 | 60.249 | End: 791 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
|---|
| Sequence | TCCTTTTTCCGCAGCCTTCT | GGTGGTCTGGAACAAACACG | Start: 129 |
| Tm | 59.89 | 59.339 | End: 391 |
| Size | 20 | 20 | 263 |
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