Primer Details for TRINITY_DN77091_c0_g1_i1 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTGAGAAAAATGAAAGAACATGGA | TGCTAGTGGATAAGTGTTTCAGA | Start: 57 |
| Tm | 60.015 | 57.261 | End: 209 |
| Size | 26 | 23 | 153 |
| Primer 2 details |
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| Sequence | TCCCTGAGAAAAATGAAAGAACATGG | TGCTAGTGGATAAGTGTTTCAGA | Start: 56 |
| Tm | 60.015 | 57.261 | End: 209 |
| Size | 26 | 23 | 154 |
| Primer 3 details |
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| Sequence | ACCACAGTTTTAACTCAAAATCCACA | TGCTAGTGGATAAGTGTTTCAGA | Start: 16 |
| Tm | 59.846 | 57.261 | End: 209 |
| Size | 26 | 23 | 194 |
| Primer 4 details |
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| Sequence | CCCTGAGAAAAATGAAAGAACATGG | TGCTAGTGGATAAGTGTTTCAGA | Start: 57 |
| Tm | 58.839 | 57.261 | End: 209 |
| Size | 25 | 23 | 153 |
| Primer 5 details |
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| Sequence | CCCTGAGAAAAATGAAAGAACATGGA | TGGATAAGTGTTTCAGATTTGACGT | Start: 57 |
| Tm | 60.015 | 59.005 | End: 203 |
| Size | 26 | 25 | 147 |
