ssr_primer
Primer Details for TRINITY_DN77071_c1_g1_i3 |
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| (A)11catcttttattacttagg(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGGAGGTGATCCCCATCT | ATGGTTGCGGAACTCGAACT | Start: 629 |
| Tm | 60.031 | 59.965 | End: 756 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | TATTGCAGAGCCGCTCCAAA | AGATGGGGATCACCTCCGAA | Start: 474 |
| Tm | 60.036 | 60.031 | End: 648 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
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| Sequence | TTATTGCAGAGCCGCTCCAA | AGATGGGGATCACCTCCGAA | Start: 473 |
| Tm | 60.036 | 60.031 | End: 648 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
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| Sequence | TTCGGAGGTGATCCCCATCT | ACGAAGAGGGAGCATGGTTG | Start: 629 |
| Tm | 60.031 | 60.036 | End: 769 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
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| Sequence | CAGAGCCGCTCCAAAGTACA | AGATGGGGATCACCTCCGAA | Start: 479 |
| Tm | 60.037 | 60.031 | End: 648 |
| Size | 20 | 20 | 170 |
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