ssr_primer
Primer Details for TRINITY_DN77062_c2_g2_i3 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTGCGAGAAAAACCCAGC | AGCAGACCGACGGTGAAATT | Start: 617 |
| Tm | 60.04 | 59.965 | End: 754 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
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| Sequence | GCTGTGCGAGAAAAACCCAG | AGCAGACCGACGGTGAAATT | Start: 616 |
| Tm | 60.04 | 59.965 | End: 754 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
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| Sequence | GAGACGTTTCTTCGCTGTGC | AGCAGACCGACGGTGAAATT | Start: 603 |
| Tm | 59.838 | 59.965 | End: 754 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
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| Sequence | AGAGAGCTTTCGCCCACTTC | GAGTGCAGTTTCTTGTCGCC | Start: 54 |
| Tm | 60.037 | 59.764 | End: 283 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
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| Sequence | CTGTGCGAGAAAAACCCAGC | TTAAGAGCAGACCGACGGTG | Start: 617 |
| Tm | 60.04 | 59.757 | End: 759 |
| Size | 20 | 20 | 143 |
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