ssr_primer
Primer Details for TRINITY_DN77054_c0_g1_i2 |
|---|
| (A)39: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACCTGCATGGCCTCTGTTG | AAAACTGGCCCCACAGACAA | Start: 791 |
| Tm | 60.035 | 60.033 | End: 917 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TGCAGGACTTTGGGATCACC | CAACAGAGGCCATGCAGGTA | Start: 610 |
| Tm | 59.962 | 60.035 | End: 810 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | TTGTCTGTGGGGCCAGTTTT | GCTCCACATGCAGCTTTAGC | Start: 898 |
| Tm | 60.033 | 59.9 | End: 1006 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TACCTGCATGGCCTCTGTTG | GCTCCACATGCAGCTTTAGC | Start: 791 |
| Tm | 60.035 | 59.9 | End: 1006 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | GATATGATCCCCGGCCATGG | GCAACCTCCTCGTTGTGGTA | Start: 352 |
| Tm | 60.107 | 59.966 | End: 499 |
| Size | 20 | 20 | 148 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACCTGCATGGCCTCTGTTG | AAAACTGGCCCCACAGACAA | Start: 791 |
| Tm | 60.035 | 60.033 | End: 917 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TGCAGGACTTTGGGATCACC | CAACAGAGGCCATGCAGGTA | Start: 610 |
| Tm | 59.962 | 60.035 | End: 810 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | TTGTCTGTGGGGCCAGTTTT | GCTCCACATGCAGCTTTAGC | Start: 898 |
| Tm | 60.033 | 59.9 | End: 1006 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TACCTGCATGGCCTCTGTTG | GCTCCACATGCAGCTTTAGC | Start: 791 |
| Tm | 60.035 | 59.9 | End: 1006 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | GATATGATCCCCGGCCATGG | GCAACCTCCTCGTTGTGGTA | Start: 352 |
| Tm | 60.107 | 59.966 | End: 499 |
| Size | 20 | 20 | 148 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACCTGCATGGCCTCTGTTG | AAAACTGGCCCCACAGACAA | Start: 791 |
| Tm | 60.035 | 60.033 | End: 917 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TGCAGGACTTTGGGATCACC | CAACAGAGGCCATGCAGGTA | Start: 610 |
| Tm | 59.962 | 60.035 | End: 810 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | TTGTCTGTGGGGCCAGTTTT | GCTCCACATGCAGCTTTAGC | Start: 898 |
| Tm | 60.033 | 59.9 | End: 1006 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TACCTGCATGGCCTCTGTTG | GCTCCACATGCAGCTTTAGC | Start: 791 |
| Tm | 60.035 | 59.9 | End: 1006 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | GATATGATCCCCGGCCATGG | GCAACCTCCTCGTTGTGGTA | Start: 352 |
| Tm | 60.107 | 59.966 | End: 499 |
| Size | 20 | 20 | 148 |
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