Primer Details for TRINITY_DN77048_c1_g1_i1 | |||
|---|---|---|---|
| (T)22: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGTGTATGTCAGTAGACTTGA | CGCAGTGTGAGGCTCTCTTT | Start: 56 |
| Tm | 58.678 | 60.32 | End: 178 |
| Size | 24 | 20 | 123 |
| Primer 2 details | |||
| Sequence | CCAGTGTATGTCAGTAGACTTGA | CGCAGTGTGAGGCTCTCTTT | Start: 57 |
| Tm | 57.355 | 60.32 | End: 178 |
| Size | 23 | 20 | 122 |
| Primer 3 details | |||
| Sequence | TCCAGTGTATGTCAGTAGACTTG | CGCAGTGTGAGGCTCTCTTT | Start: 56 |
| Tm | 57.355 | 60.32 | End: 178 |
| Size | 23 | 20 | 123 |
| Primer 4 details | |||
| Sequence | TTCCAGTGTATGTCAGTAGACTTGA | CGCAGTGTGAGGCTCTCTTT | Start: 55 |
| Tm | 59.227 | 60.32 | End: 178 |
| Size | 25 | 20 | 124 |
| Primer 5 details | |||
| Sequence | TCCAGTGTATGTCAGTAGACTTGA | AGGCTCTCTTTCATCGTGCT | Start: 56 |
| Tm | 58.678 | 59.099 | End: 169 |
| Size | 24 | 20 | 114 |
