ssr_primer
Primer Details for TRINITY_DN77037_c2_g4_i4 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTAGACCGCGCACTAAGC | AATCTAGACCGCGCATCAGG | Start: 1147 |
| Tm | 59.969 | 59.967 | End: 1387 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | ATCTAGACCGCGCACTAAGC | ACCCGGACTGTATCGGTACA | Start: 1147 |
| Tm | 59.969 | 60.034 | End: 1344 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CCTGATGCGCGGTCTAGATT | GGGATCAAGGACAGCGGAAA | Start: 1368 |
| Tm | 59.967 | 60.035 | End: 1563 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | ATCTAGACCGCGCACTAAGC | CGCTTCGTATATCCGGCTGT | Start: 1147 |
| Tm | 59.969 | 60.039 | End: 1260 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | ATCTAGACCGCGCACTAAGC | ACGCTTCGTATATCCGGCTG | Start: 1147 |
| Tm | 59.969 | 60.039 | End: 1261 |
| Size | 20 | 20 | 115 |
(G)10caa(T)29: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTAGACCGCGCACTAAGC | AATCTAGACCGCGCATCAGG | Start: 1147 |
| Tm | 59.969 | 59.967 | End: 1387 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | ATCTAGACCGCGCACTAAGC | ACCCGGACTGTATCGGTACA | Start: 1147 |
| Tm | 59.969 | 60.034 | End: 1344 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CCTGATGCGCGGTCTAGATT | GGGATCAAGGACAGCGGAAA | Start: 1368 |
| Tm | 59.967 | 60.035 | End: 1563 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | ATCTAGACCGCGCACTAAGC | CGCTTCGTATATCCGGCTGT | Start: 1147 |
| Tm | 59.969 | 60.039 | End: 1260 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | ATCTAGACCGCGCACTAAGC | ACGCTTCGTATATCCGGCTG | Start: 1147 |
| Tm | 59.969 | 60.039 | End: 1261 |
| Size | 20 | 20 | 115 |
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