ssr_primer
Primer Details for TRINITY_DN77019_c3_g1_i4 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCACTCGCGTCAAGAAGA | GCGGCATTTTTCCACACGAT | Start: 961 |
| Tm | 59.328 | 60.109 | End: 1142 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | ATCGTGTGGAAAAATGCCGC | ACGACAAATGCCAAAGCCAA | Start: 1123 |
| Tm | 60.109 | 59.25 | End: 1232 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | TTCCACTCGCGTCAAGAAGA | TTCTGGCGGCATTTTTCCAC | Start: 961 |
| Tm | 59.328 | 59.683 | End: 1147 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | TCCACTCGCGTCAAGAAGAT | GCGGCATTTTTCCACACGAT | Start: 962 |
| Tm | 59.111 | 60.109 | End: 1142 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | GTGGAAAAATGCCGCCAGAA | ACGACAAATGCCAAAGCCAA | Start: 1128 |
| Tm | 59.683 | 59.25 | End: 1232 |
| Size | 20 | 20 | 105 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCACTCGCGTCAAGAAGA | GCGGCATTTTTCCACACGAT | Start: 961 |
| Tm | 59.328 | 60.109 | End: 1142 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | ATCGTGTGGAAAAATGCCGC | ACGACAAATGCCAAAGCCAA | Start: 1123 |
| Tm | 60.109 | 59.25 | End: 1232 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | TTCCACTCGCGTCAAGAAGA | TTCTGGCGGCATTTTTCCAC | Start: 961 |
| Tm | 59.328 | 59.683 | End: 1147 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | TCCACTCGCGTCAAGAAGAT | GCGGCATTTTTCCACACGAT | Start: 962 |
| Tm | 59.111 | 60.109 | End: 1142 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | GTGGAAAAATGCCGCCAGAA | ACGACAAATGCCAAAGCCAA | Start: 1128 |
| Tm | 59.683 | 59.25 | End: 1232 |
| Size | 20 | 20 | 105 |
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