ssr_primer
Primer Details for TRINITY_DN77014_c1_g7_i1 |
|---|
| (G)32: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCAAAAAGAAGCCACGCCC | CTGAACTGACGTCGCCGATA | Start: 477 |
| Tm | 60.039 | 59.902 | End: 683 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | TCTTCATTCCGCGGGATGAG | GGGCGTGGCTTCTTTTTGAG | Start: 242 |
| Tm | 59.895 | 60.039 | End: 496 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | CACTGCTGCCACTATCGTCA | GGGCGTGGCTTCTTTTTGAG | Start: 379 |
| Tm | 60.109 | 60.039 | End: 496 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
|---|
| Sequence | CGGCAGTGTCTGTGACGTAT | CTGAACTGACGTCGCCGATA | Start: 511 |
| Tm | 60.109 | 59.902 | End: 683 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | AAGCTGCGCGAGGAAGTTAT | ATCAGTCGGTAAAAGCGCGA | Start: 612 |
| Tm | 60.108 | 60.109 | End: 800 |
| Size | 20 | 20 | 189 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCAAAAAGAAGCCACGCCC | CTGAACTGACGTCGCCGATA | Start: 477 |
| Tm | 60.039 | 59.902 | End: 683 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | TCTTCATTCCGCGGGATGAG | GGGCGTGGCTTCTTTTTGAG | Start: 242 |
| Tm | 59.895 | 60.039 | End: 496 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | CACTGCTGCCACTATCGTCA | GGGCGTGGCTTCTTTTTGAG | Start: 379 |
| Tm | 60.109 | 60.039 | End: 496 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
|---|
| Sequence | CGGCAGTGTCTGTGACGTAT | CTGAACTGACGTCGCCGATA | Start: 511 |
| Tm | 60.109 | 59.902 | End: 683 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | AAGCTGCGCGAGGAAGTTAT | ATCAGTCGGTAAAAGCGCGA | Start: 612 |
| Tm | 60.108 | 60.109 | End: 800 |
| Size | 20 | 20 | 189 |
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