ssr_primer
Primer Details for TRINITY_DN76996_c1_g1_i1 |
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| (A)12gggcacacaaatgggcaatgaatagagaaattt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTTTCCGCCATCCAGAG | ATTTTCGATCCGCCCCAGTT | Start: 537 |
| Tm | 60.036 | 60.034 | End: 677 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
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| Sequence | GCACACACTCGATGACCTCA | ATTTTCGATCCGCCCCAGTT | Start: 493 |
| Tm | 60.038 | 60.034 | End: 677 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
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| Sequence | AACTGGGGCGGATCGAAAAT | CAGTTGCGTGTTTTCTCCCG | Start: 658 |
| Tm | 60.034 | 60.041 | End: 912 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
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| Sequence | ACACTTTCCGCCATCCAGAG | TGTGCTTGCGCTTGAATCAC | Start: 537 |
| Tm | 60.036 | 60.04 | End: 646 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
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| Sequence | GCACACACTCGATGACCTCA | TGTGCTTGCGCTTGAATCAC | Start: 493 |
| Tm | 60.038 | 60.04 | End: 646 |
| Size | 20 | 20 | 154 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTTTCCGCCATCCAGAG | ATTTTCGATCCGCCCCAGTT | Start: 537 |
| Tm | 60.036 | 60.034 | End: 677 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | GCACACACTCGATGACCTCA | ATTTTCGATCCGCCCCAGTT | Start: 493 |
| Tm | 60.038 | 60.034 | End: 677 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | AACTGGGGCGGATCGAAAAT | CAGTTGCGTGTTTTCTCCCG | Start: 658 |
| Tm | 60.034 | 60.041 | End: 912 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | ACACTTTCCGCCATCCAGAG | TGTGCTTGCGCTTGAATCAC | Start: 537 |
| Tm | 60.036 | 60.04 | End: 646 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | GCACACACTCGATGACCTCA | TGTGCTTGCGCTTGAATCAC | Start: 493 |
| Tm | 60.038 | 60.04 | End: 646 |
| Size | 20 | 20 | 154 |
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