ssr_primer
Primer Details for TRINITY_DN76979_c6_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGGGTGGTCAGCTTAGAA | TGCGAGAGAAACTGCACGAT | Start: 60 |
| Tm | 60.179 | 60.038 | End: 171 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
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| Sequence | TTTTTGTTTGGGAGACGCCG | TGCGAGAGAAACTGCACGAT | Start: 38 |
| Tm | 59.617 | 60.038 | End: 171 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | GGTAGGGGGTGGTCAGCTTA | TGCGAGAGAAACTGCACGAT | Start: 57 |
| Tm | 60.62 | 60.038 | End: 171 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
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| Sequence | GGGGGTGGTCAGCTTAGAAA | TGCGAGAGAAACTGCACGAT | Start: 61 |
| Tm | 59.303 | 60.038 | End: 171 |
| Size | 20 | 20 | 111 |
| Primer 5 details |
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| Sequence | TAGGGGGTGGTCAGCTTAGA | TGCGAGAGAAACTGCACGAT | Start: 59 |
| Tm | 59.286 | 60.038 | End: 171 |
| Size | 20 | 20 | 113 |
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