ssr_primer
Primer Details for TRINITY_DN76953_c2_g5_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGTTTAGGGGCTCATCAG | GATTGCACCCACAATCCGTG | Start: 316 |
| Tm | 59.821 | 59.829 | End: 446 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
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| Sequence | ATTGGTCCTTGCAGAGCACA | TGCGTGAATTGTGGAGGACT | Start: 22 |
| Tm | 59.889 | 59.604 | End: 265 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
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| Sequence | AGTCCTCCACAATTCACGCA | GATTGCACCCACAATCCGTG | Start: 246 |
| Tm | 59.604 | 59.829 | End: 446 |
| Size | 20 | 20 | 201 |
| Primer 4 details |
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| Sequence | GTCCTTGCAGAGCACAGAGA | TGCGTGAATTGTGGAGGACT | Start: 26 |
| Tm | 59.68 | 59.604 | End: 265 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
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| Sequence | TTGGTCCTTGCAGAGCACAG | TGCGTGAATTGTGGAGGACT | Start: 23 |
| Tm | 60.536 | 59.604 | End: 265 |
| Size | 20 | 20 | 243 |
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