ssr_primer
Primer Details for TRINITY_DN76928_c3_g4_i1 |
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| (T)35: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGTCACAAATGGACGCA | ATCCAAAGGGCGAAGAAGGC | Start: 223 |
| Tm | 60.179 | 60.681 | End: 464 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
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| Sequence | ACCAGTCACAAATGGACGCA | TCCAAAGGGCGAAGAAGGC | Start: 223 |
| Tm | 60.179 | 60.303 | End: 463 |
| Size | 20 | 19 | 241 |
| Primer 3 details |
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| Sequence | GCCTTCTTCGCCCTTTGGAT | GCAGGTTGGGATGATGAATGC | Start: 445 |
| Tm | 60.681 | 59.932 | End: 586 |
| Size | 20 | 21 | 142 |
| Primer 4 details |
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| Sequence | TACCAGTCACAAATGGACGCA | ATCCAAAGGGCGAAGAAGGC | Start: 222 |
| Tm | 59.929 | 60.681 | End: 464 |
| Size | 21 | 20 | 243 |
| Primer 5 details |
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| Sequence | CCAGTCACAAATGGACGCATC | ATCCAAAGGGCGAAGAAGGC | Start: 224 |
| Tm | 59.869 | 60.681 | End: 464 |
| Size | 21 | 20 | 241 |
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