ssr_primer
Primer Details for TRINITY_DN76923_c1_g1_i5 |
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| (A)10caagagacg(A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGGCCAATGAACTCGTC | CGGCCATTTACGTTGCCAAA | Start: 4312 |
| Tm | 60.036 | 60.039 | End: 4540 |
| Size | 20 | 20 | 229 |
| Primer 2 details |
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| Sequence | TCCAGGCCAATGAACTCGTC | CACCCGTCTTTCACACCTGA | Start: 4312 |
| Tm | 60.036 | 59.894 | End: 4442 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | GCGGACTCCTTCAGGATCAG | CGGCCATTTACGTTGCCAAA | Start: 4407 |
| Tm | 59.896 | 60.039 | End: 4540 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
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| Sequence | AAATGTCTCTAGGGCGGCAC | CACAGGCACTCAGGGCTTAA | Start: 1340 |
| Tm | 60.108 | 59.963 | End: 1542 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
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| Sequence | TCAGGTGTGAAAGACGGGTG | CGGCCATTTACGTTGCCAAA | Start: 4423 |
| Tm | 59.894 | 60.039 | End: 4540 |
| Size | 20 | 20 | 118 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGGCCAATGAACTCGTC | CGGCCATTTACGTTGCCAAA | Start: 4312 |
| Tm | 60.036 | 60.039 | End: 4540 |
| Size | 20 | 20 | 229 |
| Primer 2 details |
|---|
| Sequence | TCCAGGCCAATGAACTCGTC | CACCCGTCTTTCACACCTGA | Start: 4312 |
| Tm | 60.036 | 59.894 | End: 4442 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | GCGGACTCCTTCAGGATCAG | CGGCCATTTACGTTGCCAAA | Start: 4407 |
| Tm | 59.896 | 60.039 | End: 4540 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | AAATGTCTCTAGGGCGGCAC | CACAGGCACTCAGGGCTTAA | Start: 1340 |
| Tm | 60.108 | 59.963 | End: 1542 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TCAGGTGTGAAAGACGGGTG | CGGCCATTTACGTTGCCAAA | Start: 4423 |
| Tm | 59.894 | 60.039 | End: 4540 |
| Size | 20 | 20 | 118 |
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