ssr_primer
Primer Details for TRINITY_DN76914_c0_g2_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACCCTATGTCCACGTACG | TTTGAGGTTGGGGTTGCAGT | Start: 113 |
| Tm | 59.971 | 60.033 | End: 319 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
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| Sequence | CGACCCTATGTCCACGTACG | TGTGCTTTTGAGGTTGGGGT | Start: 113 |
| Tm | 59.971 | 60.033 | End: 325 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
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| Sequence | AAAGCCCCCAGTATTGCCTC | GGAGACAAGTTCCTCGGCAA | Start: 762 |
| Tm | 60.033 | 59.965 | End: 892 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
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| Sequence | AGGCCGAACCAAACTGTAGG | GGAGACAAGTTCCTCGGCAA | Start: 738 |
| Tm | 59.964 | 59.965 | End: 892 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
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| Sequence | TTCCCGCTAAGGACAGCAAG | GGGGAACATCCGTTGGCTAA | Start: 1201 |
| Tm | 60.036 | 60.035 | End: 1363 |
| Size | 20 | 20 | 163 |
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