Primer Details for TRINITY_DN76847_c3_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCTCGGTTTAAAAACAACGT | AAAAACACACAGAGAAACACTAGTT | Start: 49 |
| Tm | 59.577 | 57.551 | End: 182 |
| Size | 22 | 25 | 134 |
| Primer 2 details |
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| Sequence | TGGCTCGGTTTAAAAACAACGT | ACGCAGATACTATAGTTAATACTCGGT | Start: 49 |
| Tm | 59.577 | 59.394 | End: 234 |
| Size | 22 | 27 | 186 |
| Primer 3 details |
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| Sequence | TGGCTCGGTTTAAAAACAACGT | CGCAGATACTATAGTTAATACTCGGT | Start: 49 |
| Tm | 59.577 | 58.027 | End: 233 |
| Size | 22 | 26 | 185 |
| Primer 4 details |
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| Sequence | TGGCTCGGTTTAAAAACAACGT | ACGCAGATACTATAGTTAATACTCGG | Start: 49 |
| Tm | 59.577 | 58.027 | End: 234 |
| Size | 22 | 26 | 186 |
| Primer 5 details |
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| Sequence | CTGGCTCGGTTTAAAAACAACG | AAAAACACACAGAGAAACACTAGTT | Start: 48 |
| Tm | 58.96 | 57.551 | End: 182 |
| Size | 22 | 25 | 135 |
