ssr_primer
Primer Details for TRINITY_DN76838_c2_g2_i7 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCTGATACCTGCAGGCGT | TGGCTTCCGAGTTATTGCGT | Start: 1114 |
| Tm | 59.823 | 60.037 | End: 1226 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | ATGGTGCGGGTTCTTGAACT | ATGCCCATCTCTGCAGCAAT | Start: 2014 |
| Tm | 59.89 | 60.106 | End: 2166 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | CTTCTGATACCTGCAGGCGT | TACACATCAGCTGGCTTCCG | Start: 1114 |
| Tm | 59.823 | 60.108 | End: 1237 |
| Size | 20 | 20 | 124 |
| Primer 4 details |
|---|
| Sequence | ACATGGTGCGGGTTCTTGAA | ATGCCCATCTCTGCAGCAAT | Start: 2012 |
| Tm | 60.179 | 60.106 | End: 2166 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
|---|
| Sequence | ACACTTCACTCCAACCCTGC | AGTTTGAGCCCAGTGTGAGG | Start: 28 |
| Tm | 60.179 | 59.891 | End: 150 |
| Size | 20 | 20 | 123 |
(A)12taagatatttagtttgtctaattgaccttattgactcaagttttttttg(T)11g(T)10g(T)10g(T)10g(T)10g(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCTGATACCTGCAGGCGT | TGGCTTCCGAGTTATTGCGT | Start: 1114 |
| Tm | 59.823 | 60.037 | End: 1226 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | ATGGTGCGGGTTCTTGAACT | ATGCCCATCTCTGCAGCAAT | Start: 2014 |
| Tm | 59.89 | 60.106 | End: 2166 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | CTTCTGATACCTGCAGGCGT | TACACATCAGCTGGCTTCCG | Start: 1114 |
| Tm | 59.823 | 60.108 | End: 1237 |
| Size | 20 | 20 | 124 |
| Primer 4 details |
|---|
| Sequence | ACATGGTGCGGGTTCTTGAA | ATGCCCATCTCTGCAGCAAT | Start: 2012 |
| Tm | 60.179 | 60.106 | End: 2166 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
|---|
| Sequence | ACACTTCACTCCAACCCTGC | AGTTTGAGCCCAGTGTGAGG | Start: 28 |
| Tm | 60.179 | 59.891 | End: 150 |
| Size | 20 | 20 | 123 |
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