ssr_primer
Primer Details for TRINITY_DN76823_c5_g3_i1 |
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| (CTA)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGAAAATAATCGGCCGGC | AGAGCCCTCCTTTCTCCCAA | Start: 75 |
| Tm | 59.97 | 60.179 | End: 297 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
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| Sequence | GCTTCTTGGGGATCTCTCCG | GAGCAAAATCGCTGACACCC | Start: 473 |
| Tm | 59.894 | 59.831 | End: 664 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
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| Sequence | TTGCACCAAGTACCACACGT | GAGCAAAATCGCTGACACCC | Start: 494 |
| Tm | 60.108 | 59.831 | End: 664 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
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| Sequence | TTGGGAGAAAGGAGGGCTCT | CGGAGAGATCCCCAAGAAGC | Start: 278 |
| Tm | 60.179 | 59.894 | End: 492 |
| Size | 20 | 20 | 215 |
| Primer 5 details |
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| Sequence | GTGGAAAATAATCGGCCGGC | GAGCCCTCCTTTCTCCCAAG | Start: 75 |
| Tm | 59.97 | 59.746 | End: 296 |
| Size | 20 | 20 | 222 |
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