ssr_primer
Primer Details for TRINITY_DN76760_c0_g1_i5 |
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| (A)29: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTCCGTACACTGTTGCAA | TCCACGAAGCCTCCTCCTTA | Start: 2286 |
| Tm | 60.179 | 59.959 | End: 2403 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
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| Sequence | GACCTTCAACAGAGCACCGA | CACCATGCCAAGAGAGCTCT | Start: 2334 |
| Tm | 59.966 | 59.748 | End: 2476 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
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| Sequence | TTCCCCACGGATTGATGAGC | TTGCAACAGTGTACGGACGT | Start: 2062 |
| Tm | 60.107 | 60.179 | End: 2305 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
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| Sequence | CCTTCAACAGAGCACCGACT | CACCATGCCAAGAGAGCTCT | Start: 2336 |
| Tm | 59.966 | 59.748 | End: 2476 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
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| Sequence | ACGTCCGTACACTGTTGCAA | GAGAAAGGGGGTGATCCACG | Start: 2286 |
| Tm | 60.179 | 60.108 | End: 2417 |
| Size | 20 | 20 | 132 |
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