ssr_primer
Primer Details for TRINITY_DN76727_c4_g2_i3 |
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| (A)40: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACTGCACTTGACGCCCAAT | GCCCGTTCAGAAACAAACCC | Start: 46 |
| Tm | 59.964 | 59.968 | End: 149 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
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| Sequence | GGGTTTGTTTCTGAACGGGC | GCCGTGGAAAGGAAGGAGAA | Start: 130 |
| Tm | 59.968 | 59.964 | End: 327 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | GGGTTTGTTTCTGAACGGGC | GAAGAGGCCGTGGAAAGGAA | Start: 130 |
| Tm | 59.968 | 59.964 | End: 333 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
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| Sequence | GGGTTTGTTTCTGAACGGGC | AAGAGGCCGTGGAAAGGAAG | Start: 130 |
| Tm | 59.968 | 59.963 | End: 332 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
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| Sequence | GGAATCCCTGGCAAAGGTCA | AAAGCCAAACCAGGGTGACA | Start: 1354 |
| Tm | 59.96 | 60.033 | End: 1552 |
| Size | 20 | 20 | 199 |
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