ssr_primer
Primer Details for TRINITY_DN76580_c0_g1_i1 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAGAACGTCACCGACACCG | AATAATCAGCTCGCAGCCGA | Start: 418 |
| Tm | 60.04 | 59.895 | End: 654 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
|---|
| Sequence | ATCGGCTCGTATGCATTGGT | AATAATCAGCTCGCAGCCGA | Start: 459 |
| Tm | 59.893 | 59.895 | End: 654 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | TCGGCTCGTATGCATTGGTT | AATAATCAGCTCGCAGCCGA | Start: 460 |
| Tm | 60.108 | 59.895 | End: 654 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
|---|
| Sequence | GGAGCGACGCGACAATTTTT | ACCAATGCATACGAGCCGAT | Start: 262 |
| Tm | 60.11 | 59.893 | End: 478 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | GGAGCGACGCGACAATTTTT | AACCAATGCATACGAGCCGA | Start: 262 |
| Tm | 60.11 | 60.108 | End: 479 |
| Size | 20 | 20 | 218 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAGAACGTCACCGACACCG | AATAATCAGCTCGCAGCCGA | Start: 418 |
| Tm | 60.04 | 59.895 | End: 654 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
|---|
| Sequence | ATCGGCTCGTATGCATTGGT | AATAATCAGCTCGCAGCCGA | Start: 459 |
| Tm | 59.893 | 59.895 | End: 654 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | TCGGCTCGTATGCATTGGTT | AATAATCAGCTCGCAGCCGA | Start: 460 |
| Tm | 60.108 | 59.895 | End: 654 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
|---|
| Sequence | GGAGCGACGCGACAATTTTT | ACCAATGCATACGAGCCGAT | Start: 262 |
| Tm | 60.11 | 59.893 | End: 478 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | GGAGCGACGCGACAATTTTT | AACCAATGCATACGAGCCGA | Start: 262 |
| Tm | 60.11 | 60.108 | End: 479 |
| Size | 20 | 20 | 218 |
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