ssr_primer
Primer Details for TRINITY_DN76577_c1_g1_i2 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCACTGTGGCCCTTTTT | TTTGTGGACTCGCTCAGACC | Start: 1120 |
| Tm | 60.033 | 59.966 | End: 1251 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | TGGTCACTGTGGCCCTTTTT | ACCGTTTACTTGTGCCCCAA | Start: 1120 |
| Tm | 60.033 | 60.107 | End: 1301 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | AGGTTCGAAACACTCCCGAC | ACCGTTTACTTGTGCCCCAA | Start: 1182 |
| Tm | 59.967 | 60.107 | End: 1301 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | TACCATTGGCGTCGTAGCAG | ATCCGTTTCGTATCTCCGGC | Start: 379 |
| Tm | 60.179 | 59.968 | End: 640 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | TGGTCACTGTGGCCCTTTTT | GCATCTAATTTCCCCCGCCT | Start: 1120 |
| Tm | 60.033 | 60.179 | End: 1340 |
| Size | 20 | 20 | 221 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCACTGTGGCCCTTTTT | TTTGTGGACTCGCTCAGACC | Start: 1120 |
| Tm | 60.033 | 59.966 | End: 1251 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | TGGTCACTGTGGCCCTTTTT | ACCGTTTACTTGTGCCCCAA | Start: 1120 |
| Tm | 60.033 | 60.107 | End: 1301 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | AGGTTCGAAACACTCCCGAC | ACCGTTTACTTGTGCCCCAA | Start: 1182 |
| Tm | 59.967 | 60.107 | End: 1301 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | TACCATTGGCGTCGTAGCAG | ATCCGTTTCGTATCTCCGGC | Start: 379 |
| Tm | 60.179 | 59.968 | End: 640 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | TGGTCACTGTGGCCCTTTTT | GCATCTAATTTCCCCCGCCT | Start: 1120 |
| Tm | 60.033 | 60.179 | End: 1340 |
| Size | 20 | 20 | 221 |
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