ssr_primer
Primer Details for TRINITY_DN76556_c3_g1_i1 |
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| (T)37: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGGTGCTGGAAGTGTATC | AGATGTGGCTGGAAGTCTGC | Start: 29 |
| Tm | 60.462 | 60.036 | End: 188 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
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| Sequence | GAAAGTTCCGGGTGCTGGAA | AGATGTGGCTGGAAGTCTGC | Start: 22 |
| Tm | 60.536 | 60.036 | End: 188 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
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| Sequence | CGGGTGCTGGAAGTGTATCT | AGATGTGGCTGGAAGTCTGC | Start: 30 |
| Tm | 59.463 | 60.036 | End: 188 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | CCGGGTGCTGGAAGTGTATC | ATGTGGCTGGAAGTCTGCAA | Start: 29 |
| Tm | 60.462 | 59.889 | End: 186 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
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| Sequence | CCGGGTGCTGGAAGTGTATC | GCACTTTGGCCATCGACATG | Start: 29 |
| Tm | 60.462 | 60.179 | End: 141 |
| Size | 20 | 20 | 113 |
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