ssr_primer
Primer Details for TRINITY_DN76535_c1_g1_i1 |
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| (A)30: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCGAAGCTTGCCTACCTT | ACCTTTGGCTGCTATGACCC | Start: 2139 |
| Tm | 60.036 | 60.034 | End: 2239 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | AAGGTCGAAGCTTGCCTACC | ACCTTTGGCTGCTATGACCC | Start: 2137 |
| Tm | 60.036 | 60.034 | End: 2239 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
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| Sequence | GGTCTAGCTTGCGGACCTAC | GTGGGCGGGTTTTCCTCTTA | Start: 218 |
| Tm | 59.897 | 59.963 | End: 490 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
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| Sequence | TTGAAGGTCGAAGCTTGCCT | ACCTTTGGCTGCTATGACCC | Start: 2134 |
| Tm | 59.891 | 60.034 | End: 2239 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
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| Sequence | GGTCTAGCTTGCGGACCTAC | GAAATAGGATGCTGCAGCGC | Start: 218 |
| Tm | 59.897 | 60.041 | End: 420 |
| Size | 20 | 20 | 203 |
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