ssr_primer
Primer Details for TRINITY_DN76513_c0_g2_i4 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCGTACTCTCCGCCATCC | GTGTGCCTGAGCCTGAGATT | Start: 14 |
| Tm | 59.969 | 60.036 | End: 163 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
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| Sequence | AATCTCAGGCTCAGGCACAC | AGGGCCCGACAGAGAAAATG | Start: 144 |
| Tm | 60.036 | 60.035 | End: 381 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
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| Sequence | AATCTCAGGCTCAGGCACAC | GAAGGGCCCGACAGAGAAAA | Start: 144 |
| Tm | 60.036 | 59.964 | End: 383 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
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| Sequence | AATCTCAGGCTCAGGCACAC | ACAGAGTTTGAGCGCACTGA | Start: 144 |
| Tm | 60.036 | 59.895 | End: 404 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
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| Sequence | CCCTGTAGATGGGTGCACAG | ACAATACGCGAGCAGACACA | Start: 1025 |
| Tm | 60.108 | 60.039 | End: 1132 |
| Size | 20 | 20 | 108 |
(G)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCGTACTCTCCGCCATCC | GTGTGCCTGAGCCTGAGATT | Start: 14 |
| Tm | 59.969 | 60.036 | End: 163 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | AATCTCAGGCTCAGGCACAC | AGGGCCCGACAGAGAAAATG | Start: 144 |
| Tm | 60.036 | 60.035 | End: 381 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
|---|
| Sequence | AATCTCAGGCTCAGGCACAC | GAAGGGCCCGACAGAGAAAA | Start: 144 |
| Tm | 60.036 | 59.964 | End: 383 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | AATCTCAGGCTCAGGCACAC | ACAGAGTTTGAGCGCACTGA | Start: 144 |
| Tm | 60.036 | 59.895 | End: 404 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | CCCTGTAGATGGGTGCACAG | ACAATACGCGAGCAGACACA | Start: 1025 |
| Tm | 60.108 | 60.039 | End: 1132 |
| Size | 20 | 20 | 108 |
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