ssr_primer
Primer Details for TRINITY_DN76484_c2_g1_i1 |
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| (A)45ccccggttgtttgatttcccctc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAAGTTATGCGCGTTTGA | GGGCGTTAATGTACCCCAGT | Start: 250 |
| Tm | 60.11 | 59.746 | End: 376 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | GCCAAGTTATGCGCGTTTGA | AGGGCGTTAATGTACCCCAG | Start: 250 |
| Tm | 60.11 | 59.455 | End: 377 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
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| Sequence | GCCAAGTTATGCGCGTTTGA | GGGAAATCAAACAACCGGGG | Start: 250 |
| Tm | 60.11 | 59.394 | End: 518 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
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| Sequence | GCCAAGTTATGCGCGTTTGA | GGGGAAATCAAACAACCGGG | Start: 250 |
| Tm | 60.11 | 59.394 | End: 519 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
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| Sequence | ACGTCGGAAAGTTGGGGAAT | GGGCAAAGGTCGAAAAAGGG | Start: 318 |
| Tm | 59.601 | 59.683 | End: 585 |
| Size | 20 | 20 | 268 |
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