ssr_primer
Primer Details for TRINITY_DN76426_c6_g4_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATGGCGTTTTTGCGTTGC | AAAATGCGCGAGAGAGGAGT | Start: 10 |
| Tm | 59.697 | 59.752 | End: 152 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
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| Sequence | CCTGCGGAGTTGGAAAAGGA | ATGCCGAGTTGGGTAGTCTG | Start: 173 |
| Tm | 60.251 | 59.463 | End: 449 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
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| Sequence | CAGACTACCCAACTCGGCAT | TTGGCCATACTGAGAGGGGT | Start: 430 |
| Tm | 59.463 | 60.253 | End: 564 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | CCTGCGGAGTTGGAAAAGGA | TGCCGAGTTGGGTAGTCTGA | Start: 173 |
| Tm | 60.251 | 60.541 | End: 448 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
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| Sequence | TCAGACTACCCAACTCGGCA | TTGGCCATACTGAGAGGGGT | Start: 429 |
| Tm | 60.541 | 60.253 | End: 564 |
| Size | 20 | 20 | 136 |
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