ssr_primer
Primer Details for TRINITY_DN76412_c3_g1_i13 |
|---|
| (T)32: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGATCAGGAGGCGGGAAA | TGGATGCGAGTTGAGTGACC | Start: 1103 |
| Tm | 60.035 | 60.037 | End: 1236 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | GTTGATCAGGAGGCGGGAAA | TCTGCGCGGACAGAGAAAAT | Start: 1103 |
| Tm | 60.035 | 60.037 | End: 1282 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | TTACGAGGTTCACGAGGCAC | TCTGCGCGGACAGAGAAAAT | Start: 1154 |
| Tm | 60.039 | 60.037 | End: 1282 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | TTGTGCTCTCTATCGGACGC | AGTGTAACGGGCTGGTCATG | Start: 153 |
| Tm | 59.899 | 60.036 | End: 397 |
| Size | 20 | 20 | 245 |
| Primer 5 details |
|---|
| Sequence | AAATGGTTGCATTGCACCCC | TTGGTCGTTGCACTGTACGA | Start: 827 |
| Tm | 59.963 | 59.897 | End: 1061 |
| Size | 20 | 20 | 235 |
(T)11gttttttttg(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGATCAGGAGGCGGGAAA | TGGATGCGAGTTGAGTGACC | Start: 1103 |
| Tm | 60.035 | 60.037 | End: 1236 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | GTTGATCAGGAGGCGGGAAA | TCTGCGCGGACAGAGAAAAT | Start: 1103 |
| Tm | 60.035 | 60.037 | End: 1282 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | TTACGAGGTTCACGAGGCAC | TCTGCGCGGACAGAGAAAAT | Start: 1154 |
| Tm | 60.039 | 60.037 | End: 1282 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | TTGTGCTCTCTATCGGACGC | AGTGTAACGGGCTGGTCATG | Start: 153 |
| Tm | 59.899 | 60.036 | End: 397 |
| Size | 20 | 20 | 245 |
| Primer 5 details |
|---|
| Sequence | AAATGGTTGCATTGCACCCC | TTGGTCGTTGCACTGTACGA | Start: 827 |
| Tm | 59.963 | 59.897 | End: 1061 |
| Size | 20 | 20 | 235 |
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