ssr_primer
Primer Details for TRINITY_DN76399_c3_g1_i1 |
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| (AG)8aaaacgactcacaattgaagatttgcctcccccccc(A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGACAGGGCAAGGCAGTAA | GACAACCGCGCAAGTAACAG | Start: 89 |
| Tm | 59.963 | 60.111 | End: 343 |
| Size | 20 | 20 | 255 |
| Primer 2 details |
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| Sequence | CTGACAGGGCAAGGCAGTAA | CAGCTTAGTTGACAACCGCG | Start: 89 |
| Tm | 59.963 | 59.836 | End: 353 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
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| Sequence | CTGACAGGGCAAGGCAGTAA | TAAACCAGACTACGCGGCTC | Start: 89 |
| Tm | 59.963 | 59.828 | End: 201 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
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| Sequence | GAGCCGCGTAGTCTGGTTTA | GACAACCGCGCAAGTAACAG | Start: 182 |
| Tm | 59.828 | 60.111 | End: 343 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
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| Sequence | AGGGCAAGGCAGTAAAGCAA | GACAACCGCGCAAGTAACAG | Start: 94 |
| Tm | 60.179 | 60.111 | End: 343 |
| Size | 20 | 20 | 250 |
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