ssr_primer
Primer Details for TRINITY_DN76352_c0_g2_i2 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAGAAGGAGAAGGGGGTC | AGCTGCTTTACACACACGGT | Start: 222 |
| Tm | 60.035 | 60.179 | End: 366 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
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| Sequence | AGAAGGAGAAGGGGGTCGAA | AGCTGCTTTACACACACGGT | Start: 225 |
| Tm | 59.883 | 60.179 | End: 366 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
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| Sequence | AAGAAGGAGAAGGGGGTCGA | AGCTGCTTTACACACACGGT | Start: 224 |
| Tm | 59.883 | 60.179 | End: 366 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | CAAGAAGGAGAAGGGGGTCG | AGCTGCTTTACACACACGGT | Start: 223 |
| Tm | 59.749 | 60.179 | End: 366 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | GCAAGAAGGAGAAGGGGGTC | GGTGGCCCCAAACTTGAAGA | Start: 222 |
| Tm | 60.035 | 60.469 | End: 349 |
| Size | 20 | 20 | 128 |
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