ssr_primer
Primer Details for TRINITY_DN76351_c0_g1_i5 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCCGTTGACAGCAAAATT | TGCGGAACTGTTAGTCACCC | Start: 379 |
| Tm | 59.967 | 59.966 | End: 657 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
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| Sequence | TTGATCGGCCCATGGGAAAA | CTGGGGCAAAGTCAAACTGC | Start: 32 |
| Tm | 59.96 | 59.967 | End: 214 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
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| Sequence | GGGTGACTAACAGTTCCGCA | TTTGTGAGTTCCCAGGAGCC | Start: 638 |
| Tm | 59.966 | 59.89 | End: 766 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
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| Sequence | TTGATCGGCCCATGGGAAAA | ACAATGTCCTGGGGCAAAGT | Start: 32 |
| Tm | 59.96 | 59.812 | End: 222 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
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| Sequence | CTACTTGATCGGCCCATGGG | CTGGGGCAAAGTCAAACTGC | Start: 28 |
| Tm | 60.25 | 59.967 | End: 214 |
| Size | 20 | 20 | 187 |
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