ssr_primer
Primer Details for TRINITY_DN76341_c1_g2_i5 |
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| (A)15tgaaaaaaacaaaacataaatgaaaaaacttaaaaaaat(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGCCCTGGGTTAACATC | TTGAGGCCTACGCACGAAAT | Start: 2678 |
| Tm | 59.96 | 60.037 | End: 2863 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
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| Sequence | CTTTGGGCCCTGGGTTAACA | TTGAGGCCTACGCACGAAAT | Start: 2676 |
| Tm | 60.179 | 60.037 | End: 2863 |
| Size | 20 | 20 | 188 |
| Primer 3 details |
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| Sequence | GTTACAGGGGCTTTCGCTCT | GTCCAACCAAAGCCAACTGC | Start: 251 |
| Tm | 60.036 | 60.249 | End: 460 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
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| Sequence | TGGACCTTCTTGCTTCGCAT | TGAGTCCAATGGCCCAACTG | Start: 456 |
| Tm | 59.963 | 60.251 | End: 589 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | TTGGGCCCTGGGTTAACATC | TTTGAGGCCTACGCACGAAA | Start: 2678 |
| Tm | 59.96 | 60.25 | End: 2864 |
| Size | 20 | 20 | 187 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGCCCTGGGTTAACATC | TTGAGGCCTACGCACGAAAT | Start: 2678 |
| Tm | 59.96 | 60.037 | End: 2863 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | CTTTGGGCCCTGGGTTAACA | TTGAGGCCTACGCACGAAAT | Start: 2676 |
| Tm | 60.179 | 60.037 | End: 2863 |
| Size | 20 | 20 | 188 |
| Primer 3 details |
|---|
| Sequence | GTTACAGGGGCTTTCGCTCT | GTCCAACCAAAGCCAACTGC | Start: 251 |
| Tm | 60.036 | 60.249 | End: 460 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | TGGACCTTCTTGCTTCGCAT | TGAGTCCAATGGCCCAACTG | Start: 456 |
| Tm | 59.963 | 60.251 | End: 589 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | TTGGGCCCTGGGTTAACATC | TTTGAGGCCTACGCACGAAA | Start: 2678 |
| Tm | 59.96 | 60.25 | End: 2864 |
| Size | 20 | 20 | 187 |
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