Primer Details for TRINITY_DN76335_c0_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTCTCTTGTTTTGAACCAAACGA | TACACGCCGTCTTGTCACTC | Start: 43 |
| Tm | 60.049 | 60.04 | End: 165 |
| Size | 25 | 20 | 123 |
| Primer 2 details |
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| Sequence | TGTTCTCTTGTTTTGAACCAAACGA | CGTCTTGTCACTCGACGTCA | Start: 43 |
| Tm | 60.049 | 60.042 | End: 158 |
| Size | 25 | 20 | 116 |
| Primer 3 details |
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| Sequence | TGTTCTCTTGTTTTGAACCAAACG | TACACGCCGTCTTGTCACTC | Start: 43 |
| Tm | 58.842 | 60.04 | End: 165 |
| Size | 24 | 20 | 123 |
| Primer 4 details |
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| Sequence | TGTTCTCTTGTTTTGAACCAAACG | CGTCTTGTCACTCGACGTCA | Start: 43 |
| Tm | 58.842 | 60.042 | End: 158 |
| Size | 24 | 20 | 116 |
| Primer 5 details |
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| Sequence | TGTTCTCTTGTTTTGAACCAAACGA | GTACACGCCGTCTTGTCACT | Start: 43 |
| Tm | 60.049 | 60.318 | End: 166 |
| Size | 25 | 20 | 124 |
