Primer Details for TRINITY_DN76310_c2_g5_i1 |
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| (T)10ggaatttctg(T)10gttcggtagatttacgagctcgtgtacaaaaaccgaacaaaa(T)15caaaaaaa(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGCTCGTGTACAAAAACCG | TCAACTTTTTGACTGATTCCGATGT | Start: 58 |
| Tm | 60.137 | 59.469 | End: 232 |
| Size | 21 | 25 | 175 |
| Primer 2 details |
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| Sequence | CGAGCTCGTGTACAAAAACCG | CAACTTTTTGACTGATTCCGATGT | Start: 58 |
| Tm | 60.137 | 58.238 | End: 231 |
| Size | 21 | 24 | 174 |
| Primer 3 details |
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| Sequence | CGAGCTCGTGTACAAAAACCG | TCAACTTTTTGACTGATTCCGATG | Start: 58 |
| Tm | 60.137 | 57.999 | End: 232 |
| Size | 21 | 24 | 175 |
| Primer 4 details |
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| Sequence | CGAGCTCGTGTACAAAAACCG | TTCAACTTTTTGACTGATTCCGATGT | Start: 58 |
| Tm | 60.137 | 59.961 | End: 233 |
| Size | 21 | 26 | 176 |
| Primer 5 details |
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| Sequence | GCTCGTGTACAAAAACCGAACA | TCAACTTTTTGACTGATTCCGATGT | Start: 61 |
| Tm | 59.969 | 59.469 | End: 232 |
| Size | 22 | 25 | 172 |
